Phosphorylation of the budgerigar fledgling disease virus major capsid protein VP1
نویسندگان
چکیده
منابع مشابه
Budgerigar fledgling disease (papovavirus) in pet birds.
References 5. Higuchi R: 1989, Rapid, efficient DNA extraction for PCR from cells or blood. Amplifications 2:1-3. 6. Kibenge FSB, Jackwood DJ, Mercado CC: 1990, Nucleotide sequence analysis of genome segment A of infectious bursal disease virus. J Gen Virol 71:569-577. 7. Kusukawa N, Uemori T, Asada K, Kato I: 1990, Rapid and reliable protocol for direct sequencing of material amplified by the ...
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Amino acid analysis of [3H]proline-labeled polyomavirus major capsid protein VP1 by two-dimensional paper chromatography of the acid-hydrolyzed protein revealed the presence of 3H-labeled hydroxyproline. Addition of the proline analog L-azetidine-2-carboxylic acid to infected mouse kidney cell cultures prevented or greatly reduced hydroxylation of proline in VP1. Immunofluorescence analysis per...
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SV40 is a small, non enveloped DNA virus with an icosahedral capsid of 45 nm. The outer shell is composed of pentamers of the major capsid protein, VP1, linked via their flexible carboxy-terminal arms. Its morphogenesis occurs by assembly of capsomers around the viral minichromosome. However the steps leading to the formation of mature virus are poorly understood. Intermediates of the assembly ...
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We have expression-cloned in Escherichia coli the major polyoma virus capsid protein, VP1. Under the inducible control of the hybrid tac promoter, VP1 constituted between 2 and 3% of the total host cell protein. The expressed VP1 was purified to near homogeneity with initial yields to 10%. Optimal expression was temperature-dependent, and significant intracellular degradation could be demonstra...
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The avian polyomavirus budgerigar fledgling disease virus (BFDV) encodes an unusual set of four agnoproteins in its late upstream region. Of the two pairs of these proteins, which overlap each other in two different reading frames, the p(L1)-promoted agnoprotein-1a (agno-1a) is the dominant species and is able to support virus propagation in the absence of the other three polypeptides. Viral BF...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1992
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.66.7.4551-4555.1992